613 research outputs found
The decays h+- -> W-+ h0(a0) within an extension of the MSSM with one complex Higgs triplet
The vertex H+-W-+h0, involving the gauge bosons W-+, the charged (H+-) and
the lightest neutral (h0) Higgs bosons, arises within the context of many
extensions of the SM, and it can be used to probe the Higgs sector of such
extensions via the decay H+- -> W+- h0. We discuss the strength of this vertex
for an extension of the MSSM with an additional complex Higgs triplet. By using
this model, we find regions of the parameter space where the decay H+- -> W+-
h0 is not only kinematically allowed, but it also becomes an important decay
mode and in some cases the dominant one.Comment: 10 figure
Store-Operated Calcium Entry in Breast Cancer Cells Is Insensitive to Orai1 and STIM1 N-Linked Glycosylation.
N-linked glycosylation is a post-translational modification that affects protein function, structure, and interaction with other proteins. The store-operated Ca2+ entry (SOCE) core proteins, Orai1 and STIM1, exhibit N-glycosylation consensus motifs. Abnormal SOCE has been associated to a number of disorders, including cancer, and alterations in Orai1 glycosylation have been related to cancer invasiveness and metastasis. Here we show that treatment of non-tumoral breast epithelial cells with tunicamycin attenuates SOCE. Meanwhile, tunicamycin was without effect on SOCE in luminal MCF7 and triple negative breast cancer (TNBC) MDA-MB-231 cells. Ca2+ imaging experiments revealed that expression of the glycosylation-deficient Orai1 mutant (Orai1N223A) did not alter SOCE in MCF10A, MCF7 and MDA-MB-231 cells. However, expression of the non-glycosylable STIM1 mutant (STIM1N131/171Q) significantly attenuated SOCE in MCF10A cells but was without effect in SOCE in MCF7 and MDA-MB-231 cells. In non-tumoral cells impairment of STIM1 N-linked glycosylation attenuated thapsigargin (TG)-induced caspase-3 activation while in breast cancer cells, which exhibit a smaller caspase-3 activity in response to TG, expression of the non-glycosylable STIM1 mutant (STIM1N131/171Q) was without effect on TG-evoked caspase-3 activation. Summarizing, STIM1 N-linked glycosylation is essential for full SOCE activation in non-tumoral breast epithelial cells; by contrast, SOCE in breast cancer MCF7 and MDA-MB-231 cells is insensitive to Orai1 and STIM1 N-linked glycosylation, and this event might participate in the development of apoptosis resistance
Polycyclic aromatic hydrocarbons in the dwarf galaxy IC 10
Infrared observations from the Spitzer Space Telescope archive are used to
study the dust component of the interstellar medium in the IC~10 irregular
galaxy. Dust distribution in the galaxy is compared to the distributions of
H and [SII] emission, neutral hydrogen and CO clouds, and ionizing
radiation sources. The distribution of polycyclic aromatic hydrocarbons (PAH)
in the galaxy is shown to be highly non-uniform with the mass fraction of these
particles in the total dust mass reaching 4%. PAHs tend to avoid bright HII
regions and correlate well with atomic and molecular gas. This pattern suggests
that PAHs form in the dense interstellar gas. We propose that the significant
decrease of the PAH abundance at low metallicity is observed not only globally
(at the level of entire galaxies), but also locally (at least, at the level of
individual HII regions). We compare the distribution of the PAH mass fraction
to the distribution of high-velocity features, that we have detected earlier in
wings of H and SII lines, over the entire available galaxy area. No
conclusive evidence for shock destruction of PAHs in the IC~10 galaxy could be
found.Comment: Accepted for publication in Astronomy Report
Metal-THINGS: The association and optical characterization of SNRs with HI holes in NGC 6946
NGC~6946, also known as the `Fireworks' galaxy, is an unusual galaxy that
hosts a total of 225 supernova remnant (SNR) candidates, including 147
optically identified with high [SII]/Ha line ratios. In addition, this galaxy
shows prominent HI holes, which were analyzed in previous studies. Indeed, the
connection between SNRs and HI holes together with their physical implications
in the surrounding gas is worth of attention. This paper explores the
connection between the SNRs and the HI holes, including an analysis of their
physical link to observational optical properties inside and around the rims of
the holes, using new integral field unit (IFU) data from the Metal-THINGS
survey. We present an analysis combining previously identified HI holes, SNRs
candidates, and new integral field unit (IFU) data from Metal-THINGS of the
spiral galaxy NGC 6946. We analyze the distributions of the oxygen abundance,
star formation rate surface density, extinction, ionization, diffuse ionized
gas, and the Baldwin-Phillips-Terlevich classification throughout the galaxy.
By analyzing in detail the optical properties of the 121 previously identify HI
holes in NGC 6946, we find that the SNRs are concentrated at the rims of the HI
holes. Furthermore, our IFU data shows that the star formation rate and
extinction are enhanced at the rims of the holes. To a lesser degree, the
oxygen abundance and ionization parameter show hints of enhancement on the rims
of the holes.
Altogether, this provides evidence of induced star formation taking place at
the rims of the holes, whose origin can be explained by the expansion of
superbubbles created by multiple supernova explosions in large stellar clusters
dozens of Myr ago.Comment: Accepted by A&
Association of the microsatellite in the 3' untranslated region of the CD154 gene with rheumatoid arthritis in females from a Spanish cohort: a case-control study
CD40–CD154 interaction is an important mediator of inflammation and has been implicated in T helper type 1-mediated autoimmune diseases including rheumatoid arthritis (RA). Linkage studies have shown association of markers in the proximity of the CD154 gene. In the present work we investigated whether specific allele variants of the microsatellite in the 3' UTR of the CD154 gene might modulate the risk of RA. The study, in a case-control setting, included 189 patients and 150 healthy controls from the Canary Islands, Spain. The 24CAs allele was less represented in female patients than in controls (0.444 in controls versus 0.307 in patients, P = 0.006, odds ratio (OR) 0.556, 95% confidence interval (CI) 0.372 to 0.831) but not in males (0.414 versus 0.408), and only when homozygous (P = 0.012; OR 0.35, 95% CI 0.16 to 0.77). We also verified that CD154 association with RA was independent of human leukocyte antigen (HLA) phenotype. A further functional study showed that after stimulation anti-CD3, CD154 mRNA was more stable in CD4+ T lymphocytes from patients with RA bearing the 24CAs allele (mRNA half-life 208 minutes) than in patients without the 24CAs allele (109 minutes, P = 0.009). However, a lower percentage of CD154+CD4+ T lymphocytes was seen in freshly isolated peripheral blood mononuclear cells from patients carrying 24CAs alleles (mean 4.28 versus 8.12; P = 0.033), and also in CD4+ T lymphocytes stimulated with anti-CD3 (median 29.40 versus 47.60; P = 0.025). These results were concordant with the smaller amounts of CD154 mRNA isolated from stimulated T lymphocytes with 24CAs alleles. The CD154 microsatellite therefore seems to affect the expression of the gene in a complex manner that implies not only mRNA stability. These data suggest that the CD154 microsatellite contributes to the regulation of mRNA and protein expression, although further studies will be necessary to elucidate its role in disease predisposition
EFHB is a Novel Cytosolic Ca2+ Sensor That Modulates STIM1-SARAF Interaction
Background/Aims: STIM1 and Orai1 are the key components of store-operated Ca2+ entry (SOCE). Among the proteins involved in the regulation of SOCE, SARAF prevents spontaneous activation of SOCE and modulates STIM1 function. Methods: Cytosolic Ca2+ mobilization was estimated in fura-2-loaded cells using an epifluorescence inverted microscope. STIM1 interaction with Orai1, EFHB (EF-hand domain family member B, also known as CFAP21) and SARAF was detected by immunoprecipitation followed by Western blotting using specific antibodies. The involvement of EFHB in the translocation of NFAT to the nucleus was detected by confocal microscopy. Results: Here, we report the identification of EFHB as a new SOCE regulator. EFHB interacts with STIM1 upon store depletion and dissociates through a Ca2+-dependent mechanism. RNAi-mediated silencing as well as overexpression studies revealed that EFHB plays a relevant role in the interaction of STIM1 and Orai1 upon store depletion, the activation of SOCE and NFAT translocation from the cytosol to the nucleus. Silencing EFHB expression abolished the dissociation of SARAF from STIM1, which indicates that EFHB might play an important role in the dynamic interaction between both proteins, which is relevant for the activation of Orai1 channels upon Ca2+ store depletion and their subsequent modulation via slow Ca2+-dependent inactivation. Conclusion: Our results indicate that EFHB is a new SOCE regulator that modulates STIM1-SARAF interaction
Azimuthal asymmetry in the risetime of the surface detector signals of the Pierre Auger Observatory
The azimuthal asymmetry in the risetime of signals in Auger surface detector
stations is a source of information on shower development. The azimuthal
asymmetry is due to a combination of the longitudinal evolution of the shower
and geometrical effects related to the angles of incidence of the particles
into the detectors. The magnitude of the effect depends upon the zenith angle
and state of development of the shower and thus provides a novel observable,
, sensitive to the mass composition of cosmic rays
above eV. By comparing measurements with predictions from
shower simulations, we find for both of our adopted models of hadronic physics
(QGSJETII-04 and EPOS-LHC) an indication that the mean cosmic-ray mass
increases slowly with energy, as has been inferred from other studies. However,
the mass estimates are dependent on the shower model and on the range of
distance from the shower core selected. Thus the method has uncovered further
deficiencies in our understanding of shower modelling that must be resolved
before the mass composition can be inferred from .Comment: Replaced with published version. Added journal reference and DO
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